




Biol 189T -
Industrial Biotechnology
Lecture
Schedule
Fall 2000
Tentative
Course Schedule:
Lecture
Day
|
Date
|
Reading
Chapter
in Demain
|
Topic
|
Assignments
|
1
|
8/28
|
1
|
Cultures
|
|
2
|
8/30
|
20
|
Growth
|
|
|
9/4
|
Labor Day
|
|
|
3
|
9/6
|
12
|
Continuous culture
|
Set up groups
|
4
|
9/11
|
4
|
Small scale fermentations
|
|
5
|
9/13
|
18
|
Instrumentation
|
|
6
|
9/18
|
|
Work on reports
|
|
7
|
9/20
|
17
|
Raw materials
|
Progress reports
|
8
|
9/25
|
3
|
Preservation and inoculum
|
|
9
|
9/27
|
19
|
Scale up strategies
|
Lab notebooks due
|
10
|
10/2
|
|
Work on reports
|
|
11
|
10/4
|
6
|
System improvement
|
Progress reports
|
12
|
10/9
|
|
Winery Tour
|
|
13
|
10/11
|
20, 8
|
Data analysis & Strain improvement
|
|
14
|
10/16
|
5, 7, 9
|
Solid state methods and immobilization
|
|
15
|
10/18
|
9, 10, 11
|
Thermophiles, Anaerobic methods
|
|
16
|
10/23
|
MIDTERM
|
|
MIDTERM
|
17
|
10/25
|
13
|
Biocatalysis and biotransformations
|
Progress reports
|
18
|
10/30
|
16, 15
|
Insect cells and plants
|
|
19
|
11/1
|
15, 14
|
Mammalian cells
|
|
20
|
11/6
|
21, 22
|
Small molecules, Proteins
|
|
21
|
11/8
|
23
|
Economics
|
Progress reports
|
22
|
11/13
|
25, 26
|
Safety and intellectual property
|
|
23
|
11/15
|
26, 28
|
Quality control
|
|
24
|
11/20
|
2, 52
|
Bioprospecting
|
Lab notebooks due
|
|
11/22
|
Thanksgiving
|
|
|
25
|
11/27
|
47, 48, 49
|
Recombinant DNA applications
|
|
26
|
11/29
|
45, 47
|
Recombinant DNA applications
|
|
27
|
12/4
|
|
Work on projects
|
|
28
|
12/6
|
50, 51
|
Metabolic engineering, PHA
|
Strategic reports due
|
29
|
12/11
|
55
|
Bioremediation
|
Strategic report presentations
|
30
|
12/13
|
59
|
Antibiotics
|
|
|
12/15
|
|
|
Lab note books due
|
Schedule is tentative, any changes will be announced in
class.

Lab
Calendar
August 2000
Sun - READINGS
From Demain
|
Mon
|
Tue
|
Wed
|
Thu
|
Fri
|
Sat
|
|
|
1
|
2
|
3
|
4
|
5
|
6
|
7
|
8
|
9
|
10
|
11
|
12
|
13
|
14
|
15
|
16
|
17
|
18
|
19
|
20
|
21
|
22
|
23
|
24
|
25
|
26
|
27
Ch
1, 20
|
28
sterile techniques
|
29
|
30
|
31
|
|
|
September 2000
Sun - READING From
Demain
|
Mon
|
Tue
|
Wed
|
Thu
|
Fri
|
Sat
|
|
|
|
|
|
1
|
2
|
3
Ch
12
|
4
Labor Day
|
5
|
6
Strategic Reports Groups
organized
|
7
|
8
|
9
|
10
Ch
4, 18
|
11
growth curve
|
12
|
13
|
14
|
15
|
16
|
17
Ch 17
|
18
intro to the bioreactor, media,
sterilization
|
19
|
20
*progress reports*
|
21
Seed culture start,
reactor prep
|
22
start reactor
|
23
|
24
Ch 3, 19
|
25
cell storage methods
Quiz
1
|
26
|
27
*lab note
books due*
|
28
|
29
|
30
|
October 2000
Sun -
READING From Demain
|
Mon
|
Tue
|
Wed
|
Thu
|
Fri
|
Sat
|
1
Ch
6
|
2
media, sterilize
|
3
prep bioreactor
seed culture |
4
*progress reports*
start reactor |
5
induce GFP
|
6
harvest
|
7
|
8
Ch 20, 8
|
9
cell lysis methods
Winery Tour
Quiz
2
|
10
|
11 |
12
|
13
|
14
|
15
Ch
5, 7, 9, 10, 11
|
16
GFP purify/quantify
|
17
|
18 |
19
insect starter culture
|
20
|
21
|
22
Ch 13
|
23
*midterm*
insect culture media
|
24
reactor prep
|
25
start reactor
*progress reports*
|
26
|
27
|
28
|
29
Ch 15, 16
|
30
larvae
|
31
infect cells, feed larvae
|
|
|
|
|
November 2000
Sun - READING From
Demain
|
Mon
|
Tue
|
Wed
|
Thu
|
Fri
|
Sat
|
Ch
14, 15
|
|
|
1
feed
|
2
|
3
harvest
|
4
|
5
Ch 21, 22, 23
|
6
GFP purify/quantify
|
7
insulin bioreactor
|
8
*progress reports*
|
9
|
10
|
11
|
12
Ch
25, 26, 28
|
13
insulin purification
Quiz
5
|
14
|
15 |
16 |
17 |
18
|
19
Ch
2, 52
|
20
Insulin ELISA
Lab notebooks due |
21
|
22
Thanksgiving
|
23
Thanksgiving
|
24
Thanksgiving
|
25
|
26
Ch
45, 47, 48, 49
|
27
trip
|
28
|
29
|
30
|
|
|
December 2000
Sun -
READING From Demain
|
Mon
|
Tue
|
Wed
|
Thu
|
Fri
|
Sat
|
|
|
|
|
|
1
|
2
|
3
Ch 50 & 51
|
4
GC and FPLC
analysis
|
5
|
6
*written strategic
reports due*
|
7
|
8
Quiz
6
|
9
|
10
Ch
55, 59
|
11
*strategic reports
presentations*
|
12
|
13
|
14
|
15
*lab notebooks due*
|
16
|
17
|
18
|
19
|
20
FINAL
|
21
|
22
|
23
|
24
|
25
|
26
|
27
|
28
|
29
|
30
|
31
|
|
|
|
|
|
|

Reports
Report Due Dates
Date |
Task |
September 6, 2000 |
Strategic Reports Groups
organized |
September 20, 2000 |
Progress
reports |
October 4, 2000 |
Progress
reports |
October 25, 2000 |
Progress
reports |
November 8, 2000 |
Progress
reports |
December 6, 2000 |
Written strategic
reports due |
December 11, 2000 |
Strategic reports presentations |

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