Publications
Gousset K. and Tovar J. (2026). From LCM to Multi-Omics: A Multidimensional Pipeline for High-Confidence Mapping of the Filopodia Proteome. In preparation.
Tovar J. and Gousset K. (2026). Decoy-Free False Discovery Rate Estimation Using Multiple Statistical Models in the Sage Proteomics Search Engine. In preparation.
Savala D. and Gousset K. (2026). The role of Arf1 in the formation of tunneling nanotubes in neuronal CAD cells. In preparation.
Perez, A.R., Tovar J., and Gousset K. (2026). Myosin-X Acts Upstream of L-Plastin to Drive Stress-Induced Tunneling Nanotubes. Cells. 2026 Jan 24;15(3):224. doi: 10.3390/cells15030224.
Gordon A, Gousset K. (2021). Utilization of laser capture microdissection coupled to mass spectrometry to uncover the proteome of cellular protrusions. Methods Mol Biol. 2021;2259:25-45. PubMed.
Gousset K., Gordon A., Kannan S., and Tovar J. (2019). A novel Microproteomic Approach Using Laser Capture Microdissection to Study Cellular Protrusions. Int. J. Mol. Sci. 2019, 20, 1172. PubMed
Sartori-Rupp A., Cordero D., Pepe A., Gousset K., Delage E., Corroyer-Dulmont S., Schmitt C., Krijnse-Locker J., and Zurzolo C. (2019). Correlative cryo-electron microscopy reveals the structure of TNTs in neuronal cells. Nat Commun. 2019;10(1):342. PubMed
Uhl J., Gujarathi S., Waheed A.A., Gordon A., Freed E.O., and Gousset K. (2018). Myosin-X is essential to the intercellular spread of HIV-1 Nef through tunneling nanotubes. Journal of Cell Communication and Signaling. PubMed
Gordon A., Kannan S., and Gousset K. (2018). A novel cell fixation method that greatly enhances protein identification in microproteomic studies using laser capture microdissection and mass spectrometry. Proteomics. PubMed
Tanveer A., Ariazi J., Benowitz A., Biasi V., Den Boer M., Cherqui S., Douillet N., Eugenin E.A., Favre D., Goodman S., Gousset K., Hanein D., Israel D., Kimura S., Kirkpatrick R., Kuhn N., Jeong C., Lou E., Mailliard R., Maio S., Okafo G., Osswald M., Pasquier J., Polak R., Pradel G., Rooij B., Schaeffer P., Skeberdis A., Smith I., Volkmann N., Wu Z. and Zurzolo C. (2017). Tunneling Nanotubes and Gap Junctions – their role in long-range intercellular communication during development, health, and disease. Front. Mol. Neurosci.. PubMed
Gousset K., Marzo L., Commere P.H., and Zurzolo C. (2013). Myo10 is a key regulator of TNT formation in neuronal cells. J Cell Sci. 126(Pt 19):4424-35. PubMed
Marzo L., Gousset K., and Zurzolo C. (2012). Multifaceted roles of tunneling nanotubes in intercellular communication. Front Physiol. 3:72. PubMed
Langevin C., Gousset K., Costanzo M. and Zurzolo C. (2010). Characterization of the role of dendritic cells in prion transfer to primary neurons. Biochem J. 431(2):189-98. PubMed
Bennett A.E., Narayan K., Shi D., Hartnell L.M., Gousset K., He H., Lowekamp B.C., Yoo T.S., Bliss D., Freed E.O., and Subramaniam S. (2009). Ion-Abrasion scanning electron microscopy reveals surface-connected tubular conduits in HIV-infected macrophages. PLoS Pathog. 5(9). PubMed
Gousset K. and Zurzolo C. (2009). Tunnelling nanotubes: a highway for prion spreading? Prion. 3(2):94-98. PubMed
Gousset K., Schiff E., Langevin C., Marijanovic Z., Caputo A., Browman D.T., Chenouar N., de Chaumont F., Martino A., Enninga J., Olivo-Marin J-C., Männel D., and Zurzolo C. (2009). Prions Hijack tunnelling nanotubes for intercellular spread. Nat. Cell. Biol. 11(3):328-336. PubMed
Schiff E, Campana V, Tivodar S, Lebreton S, Gousset K., Zurzolo C. (2008). Coexpression of wild-type and mutant prion proteins alters their cellular localization and partitioning into detergent-resistant membranes. Traffic. 9(7):1101-1115. PubMed
Gousset K., Ablan S.D., Coren L.V., Ono A., Soheilian F., Nagashima K., Ott D.E., Freed E.O. (2008). Real-time visualization of HIV-1 GAG trafficking in infected macrophages. PLoS Pathog. 4(3):e1000015. PubMed
Wu C.K., Gousset K., and Hughes S.H. (2007). Targeting expression to the endoplasmic reticulum improves the protein folding of human telomerase reverse transcriptase. Protein Expr Purif. 56(1):8-19. PubMed
Liu F., Stephen A.G., Adamson C.S., Gousset K., Aman M.J., Freed E.O., Fisher R.J. and Burke T.R. Jr. (2006). Hydrazone- and hydrazide-containing N-substituted glycines as peptoid surrogates for expedited library synthesis. Org. Lett. 8(22):5165-5168. PubMed
Shrimpton C.N., Gousset K., Tablin F., and Lopez J.A. (2004). Isolation and analysis of platelet lipid rafts. In: Platelets and Megakaryocytes. Methods Mol. Biol. 273:213-238. PubMed
Leidy C., Gousset K., Ricker J., Wolkers W.F., Tsvetkova N.M., Tablin F., and Crowe J.H. (2004). Lipid Phase Behavior and Stabilization of Domains in Membranes of Platelets. Cell Biochem Biophys. 40(2):123-148. PubMed
Sharma P., Varma R., Sarasij R.C., Ira, Gousset K., Krishnamoorthy G., Rao M., and Mayor S. (2004). Nanoscale organization of multiple GPI-anchored proteins in living cell membranes. Cell 116(4):577-589. PubMed
Gousset K., Tsvetkova N.M., Crowe J.H., and Tablin F. (2004). Important role of raft aggregation in the signaling events of cold-induced platelet activation. Biochim. Biophys. Acta 1660(1-2):7-15. PubMed
Crowe J.H., Tablin F., Wolkers W.F., Gousset K., Tsvetkova N.M. and Ricker J. (2003). Stabilization of membranes in human platelets freeze-dried with trehalose. Chem. Phys. Lipids 122(1-2):41-52. PubMed
Gousset K., Wolker W.F., Tsvetkova N.M., Oliver A.E., Field C.L., Walker N.J., Crowe J.H. and Tablin F. (2002). Evidence for a physiological role for membrane rafts in human platelets. J. Cell. Physiol. 190(1):117-128. PubMed
Tablin F., Wolkers W.F., Walker N.J., Oliver A.E., Tsvetkova N.M., Gousset K., Crowe L.M., and Crowe J.H. (2001). Membrane reorganization during chilling: Implications for long-term stabilization of platelets. Cryobiology 43(2):114-123. PubMed
Invited Lectures
Gousset K. (2023) Tunneling nanotubes, their role in diseases, and development of techniques to study these subcellular structures. Department of Biological Sciences, California State University, Stanislaus.
Gousset K. (2021) Uncovering the proteomes and transcriptomes of cellular protrusions using laser capture microdissection. Department of Chemistry and Biochemistry, California State University, Long Beach.
Gousset K. (2021) Isolation of Cellular Protrusions Using Laser Capture Microdissection for Subcellular Microproteomics Studies (Canceled due to COVID). MMI Meeting on Microdissection, Micromanipulation, and Imaging. Aying, Germany.
Gousset K. (2020) Microproteomic Analysis of Laser Capture Microdissected Cell Protrusions. World Congress on In Vitro Biology, San Diego, CA.
Gousset K. (2020) What the Cell is Going On? Scientists Reveal Methods for Successful Single-Cell Omics Research. MMI Webinar.
Gousset K. (2020) Microproteomic analysis of laser capture microdissected cell protrusions. Department of Biology, California State University, Fresno.
Gousset K. (2019) Laser capture microdissection and microproteomics: uncovering the proteomes of diverse cellular protrusions. American Society for Cell Biology (ASCB) Annual Meeting, Washington, DC.
Gousset K. (2019) A Novel Cell Fixation Method that Greatly Enhances Protein Identification in Microproteomic Studies Using Laser Capture Microdissection and Mass Spectrometry. 7th International Congress on Analytical Proteomics, Caparica, Portugal.
Gousset K. (2018) Microproteomic analysis of laser capture microdissected cell protrusions. Department of Biology & Molecular Biology, Colorado State University.
Gousset K. (2017) Microproteomic analysis of laser capture microdissected cell protrusions. Department of Biology, San Francisco State University.
Gousset K. (2016) Myosin-X and HIV-1 Nef: Towards a disease state model system and marker of tunneling nanotubes. GSK, Pennsylvania.
Gousset K. (2015) TNTs, Myosin-X and mitochondria: a new paradigm in chronic and persistent disease. University of Mississippi School of Pharmacy.
Gousset K. (2015) HIV-1 Nef Induces TNTs in Macrophages through Up-regulation of Myo10. American Society for Virology Annual Meeting, London, Ontario, Canada.
Gousset K. (2015) HIV-1 Nef Induces TNTs in Macrophages through Up-regulation of Myo10. NCI Virus-Cell Interaction Section, Frederick, MD.
Gousset K. (2015) The role of Calpain-2 in Myosin X related diseases. Queen's Cancer Research Institute, Queen’s University, Kingston, ON, Canada.
Gousset K. (2015) The role of tunneling nanotubes and Myosin X in disease. Department of Genetics, School of Medicine, Stanford University.
Zurzolo C., Gousset K., and Marzo L. (2011). Mechanisms of Intercellular Trafficking of PrPsc. PRION 2011. Montreal, Quebec, Canada.
Gousset K., Marzo L. and Zurzolo C. (2011) Role of Tunneling nanotubes (TNTs) in PrPSc intercellular spreading. 1st French-Italian joint meeting on subcellular trafficking. Padua, Italy.
Gousset K., Marzo L. and Zurzolo C. (2009) Characterization of tunnelling nanotubes (TNTs) in neuronal cells. BCI Departmental retreat; La Colle sur Loup, France.
Gousset K. et al. (2008) Prions Hijack tunneling nanotubes for intercellular spread. “Imaging Host-Pathogen Interactions” symposium in Heidelberg, Germany.
Gousset K., Tsvetkova N.M., Crowe J.H., and Tablin F. (2002) Selective Partitioning of receptors and signaling molecules in raft membrane domains of human platelets. Biophys. J. 82.
Gousset K. and Tablin F. (2000) Membrane Rafts are a Key Physiological Event in Human Platelet Cold-Induced Activation. Mol. Biol. Cell 11.
Poster Presentations
Kaur, R. and Gousset K. (2025) Effect of Fixation on Immunofluorescence. 45th Annual Central California Research Symposium.
Ortiz, D. and Gousset K. (2025) Role of Lactate dehydrogenase B in formation of tunneling nanotubes. 45th Annual Central California Research Symposium.
Ramirez, A. and Gousset K. (2024) Analyzing the Role of L-Plastin in the Formation of Tunneling Nanotubes. 45th Annual Central California Research Symposium.
Pama-Ghuman, A., Stewart, K., Nishimura, E., Gousset K. (2024) Characterization of the Transcriptomes of Specific Subtypes of Cellular Protrusions Isolated by Laser Capture Microdissection. 45th Annual Central California Research Symposium.
Savala D. and Gousset K. (2023) Exploring the Role of Protein-X in Tunneling Nanotube Formation. 35th Annual CSU Biotechnology Symposium.
Pingitore F., Adams C., and Gousset K. (2022) Proteome characterization of tunneling nanotubes with dda-PASEF acquisition mode. ASMS Conference on Mass Spectrometry and Allied Topics, Minneapolis Convention Center, Minneapolis, MN
Green N. and Gousset K. (2022) Does the expression level of the protein Vanin 1 have an effect on the formation of Tunneling nanotubes in neuronal cells? 43rd Annual Central California Research Symposium.
Savala D. and Gousset K. (2022) Exploring the role of ADP-ribosylation Factor 1 (Arf1) in Tunneling Nanotube (TNT) formation. 43rd Annual Central California Research Symposium.
Putnam N. and Gousset K. (2021) Antimycin A Leads to the Upregulation of Myo10 and Tunneling Nanotube Formation. 42nd Annual Central California Research Symposium.
Azami P., and Gousset K. (2020) The Role of CD7 Protein in Tunneling Nanotube Formation. 32nd Annual CSU Biotechnology Symposium.
Ajanel J., Xiong Z., Pham A., and Gousset K. (2020) The Effect of Hydrogen Peroxide and Glutamate on Myosin-X expression and TNT Formation. 32nd Annual CSU Biotechnology Symposium.
Azami P. and Gousset K. (2020) Role of CD7 protein in Tunneling Nanotube formation in Neuronal Cells. 41st Annual Central California Research Symposium.
Ajanel J., Xiong Z., Pham A., and Gousset K. (2019) Physiological Concentrations of Hydrogen Peroxide and Glutamate, Induce Tunneling Nanotube Formation in Hela and Cad Cells. 2019 American Society for Cell Biology (ASCB) Annual Meeting, Washington, DC.
Ajanel J. and Gousset K. (2019) Physiological Concentrations Of Hydrogen Peroxide And Glutamate, Induce Tunneling Nanotube Formation In Hela Cells. 40th Annual Central California Research Symposium.
Ajanel, A., Xiong, Z., Pham, A., and Gousset, K. Chemical secreted by cancer cells, such as hydrogen peroxide and glutamate, induce tunneling nanotube formation in HeLa and neuronal CAD cells. California State University Summer Symposium, UCLA.
Trujillo A., and Gousset K. (2019) The Role Of Mitochondrial Dna In Tunneling Nanotubes Formation In Rho Cells. 40th Annual Central California Research Symposium.
Pham A., and Gousset K. (2019) Do Physiological Concentration Of Hydrogen Peroxide And Glutamate Affect The Expression Of Myosin X In Cancer Cells? 40th Annual Central California Research Symposium.
Uhl J., and Gousset K. (2018) Fluorescence In Situ Hybridization (FISH) to Study the Role of Myo10 in TNTs. 39th Annual Central California Research Symposium.
Tovar M. and Gousset K. (2017) The proteolysis of Myo10, by Calpain, under low calcium conditions is activated by its FERM domain Acyl-CoA Binding Protein Motif. ASCB Annual Meeting, Philadelphia, PA.
Tipan P. and Gousset K. (2017) Development of Pichia pastoris as a model system for Myosin X-induced filopodia formation. 29th Annual CSU Biotechnology Symposium.
Gousset K., Gordon A., and Kannan S. (2016) Laser capture microdissection and microproteomics: a novel method to study the proteomes of cell protrusions. ASCB Annual Meeting, San Francisco, CA.
Tipan P. and Gousset K. (2016) Development of Pichia pastoris as a model system for Myosin X-induced filopodia formation. ASCB Annual Meeting, San Francisco, CA.
Kannan S. and Gousset K. (2016) Microproteomic analysis of laser capture microdissected cells forming TNTs. 37th Annual Central California Research Symposium.
Kannan S. and Gousset K. (2015) Microproteomic analysis of laser capture microdissected cells forming TNTs. Stanford University Mass Spectrometry Research Applications Symposium.
Gujarathi S. and Gousset K. (2015) Myosin-X is critical for functional HIV-1 Nef induced tunneling nanotubes (TNTs). ASCB Annual Meeting, San Diego, CA.
Gujarathi S. and Gousset K. (2015) HIV-1 Nef protein induces TNT formation in macrophages by up-regulation of Myo10. American Society for Virology Annual Meeting, London, Ontario, Canada.
Han S. and Gousset K. (2015) A Myo10 c-terminal fragment associates with components of messenger ribonucleoprotein (mRNP) complexes in the core nuclear region. 36th Annual Central California Research Symposium.
Gujarathi S. and Gousset K. (2015) HIV-1 Nef protein induces TNT formation in macrophages by up-regulation of Myo10. 36th Annual Central California Research Symposium.
Gujarathi S. and Gousset K. (2015) HIV-1 Nef protein induces TNT formation in macrophages by up-regulation of Myo10. 27th Annual CSU Biotechnology Symposium.
Han S. and Gousset K. (2015) Interaction of the FERM Domain of Myo10 with the Tumor Suppressor p53. 27th Annual CSU Biotechnology Symposium.
Gujarathi S. and Gousset K. (2014) Effect of Nef protein on TNT formation in macrophages. 35th Annual Central California Research Symposium.
Gousset K., Coren L.V., Ablan S., Ott D., and Freed E.O. (2006) Visualization of HIV-1 Gag trafficking in living cells in real time using the biarsenical-tetracysteine system. Cold Spring Harbor Retroviruses Conference.
Edidin M., Gousset K., Detre C., and Gheber L. (2006) Membrane domains: Probing the floating world. Abstracts of Papers of the American Chemical Society.
Gousset K., Demirov D.G., Goila-Gaur R., Kostelansky M.S., Beach B.M., Hurley J.H., and Freed E. (2005) The Role of ESCRT-I components in endosomal sorting and HIV-1 release. HIV Drug Resistance Symposium.
Tablin F., Gousset K., and Crowe J.H. (2004) Platelet lipid raft aggregation associated with cold storage: the role of lateral phase transition. Arterioscler. Thromb. Vasc. Biol.
Gousset K., Tsvetkova N.M., Crowe John H., and Tablin F. (2001) Selective Partitioning of receptors and signaling molecules in raft membrane domains of human platelets. Mol. Biol. Cell 12.
Tablin F. and Gousset K. (2000) Membrane Rafts are a Key Physiological Event in Human Platelet Cold-Induced Activation. Blood 11.